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Blood culture bottles for transportation and recovery of anaerobic bacteria from non‐blood samples
Author(s) -
Melhus Åsa,
Tjernberg Ingela
Publication year - 2000
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1034/j.1600-0463.2000.d01-82.x
Subject(s) - anaerobic exercise , bacteria , anaerobic bacteria , bottle , blood culture , microbiology and biotechnology , food science , biology , physiology , antibiotics , materials science , genetics , composite material
Using bacterial suspensions as simulated non‐blood specimens, the capacity of three different BacT/Alert blood culture bottles for the transportation and recovery of anaerobic bacteria with different sensitivity to air was evaluated. To better assess the performance of the BacT/Alert bottles, three other liquid media specially designed for anaerobes were included in the study. Attention was paid to recovery rates in relation to species, initial bacterial concentration, and time needed for detection. Of the BacT/Alert blood culture bottles, the anaerobic FAN bottle yielded the highest recovery rates, but its performance was limited compared with chopped meat broth in tubes. This broth allowed detection of all the tested species within 48 h. Since collection and transportation of anaerobic bacteria are of major importance for a reliable culture result, improvements are necessary.