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Drought tolerance of photosynthetic electron transport under CO 2 ‐enriched and normal air in cereal species
Author(s) -
Flagella Z.,
Campanile R. G.,
Stoppelli M. C.,
De Caro A.,
Di Fonzo N.
Publication year - 1998
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.1998.1040434.x
Subject(s) - photosynthesis , cultivar , hordeum vulgare , chlorophyll fluorescence , drought stress , dehydration , photosystem ii , electron transport chain , chlorophyll , biology , drought tolerance , agronomy , poaceae , chemistry , horticulture , botany , biochemistry
The quantum yield of photosynthetic electron transport (ΦPSII), evaluated by means of chlorophyll (Chl) fluorescence analysis, has proven to be a useful screening test for drought tolerance in durum wheat ( Triticum durum Desf.). To explore the potential of this parameter further in detecting drought‐tolerant genotypes, three cereal species were studied; ΦPSII measurements were carried out under two different gas mixtures, at three points of the induction curve (to obtain the maximal ΦPSII and both the transient and steady‐state actual ΦPSII), and at three different water stress levels (moderate, severe and drastic). The species investigated were durum and bread wheat ( Triticum aestivum L.) and barley ( Hordeum vulgare L.); two cultivars per species, characterized by different levels of drought tolerance, were tested. The two gas mixtures used were normal air (21% O 2 , 0.035% CO 2 in N 2 ) to monitor the whole photosynthetic process under physiological conditions, and CO 2 enriched‐low O 2 air (1% O 2 , 5% CO 2 in N 2 ) to monitor ΦPSII reduction under stress mainly related to Calvin cycle activity. When ΦPSII related to both assimilatory and non‐assimilatory metabolism was evaluated, the cultivar differences observed under normal Air were more representative of the agronomic performance upon drought stress than under high CO 2 ‐low O 2 air. Maximal ΦPSII showed no difference among either cultivars, gas mixtures or stress levels, the efficiency of excitation capture being highly resistant to drought. The ΦPSII evaluated during the transient yielded predictable values in respect of drought tolerance for durum wheat and barley cultivars, highlighting the key role of regulatory processes such as the Mehler peroxidase reaction and possibly also cyclic electron transport, in preventing overreduction under stress. The results clearly show that when Chl fluorescence analysis is used as a parameter in plant breeding, different experimental conditions should be used depending on the physiological mechanism that is bred or selected for.