Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging | Zendy

Wenwen Jing | Zendy; Yan Wang | Zendy; Yunze Yang | Zendy; Yi Wang | Zendy; Guangzhong Ma | Zendy; Shaopeng Wang | Zendy; gjian Tao | Zendy

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Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging

Author(s) -

Wenwen Jing,

Yan Wang,

Yunze Yang,

Yi Wang,

Guangzhong Ma,

Shaopeng Wang,

gjian Tao

Publication year - 2019

Publication title -

acs nano

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.554

H-Index - 382

eISSN - 1936-086X

pISSN - 1936-0851

DOI - 10.1021/acsnano.9b02771

Subject(s) - procalcitonin , detection limit , immunoassay , dynamic range , plasmon , nanotechnology , materials science , nanoparticle , wide dynamic range , optics , chromatography , medicine , chemistry , optoelectronics , physics , antibody , immunology , sepsis

Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ∼2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of ∼25 min.

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