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Molecular cloning and characterization of an adaptor protein Shc isoform from Xenopus laevis oocytes
Author(s) -
Chesnel Franck,
Heligon Christophe,
RichardParpaillon Laurent,
Boujard Daniel
Publication year - 2003
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(03)00058-3
Subject(s) - xenopus , biology , signal transducing adaptor protein , oocyte , grb2 , microbiology and biotechnology , guanine nucleotide exchange factor , signal transduction , embryo , genetics , gene
Abstract In order to gain further insight into IGF‐1 receptor signaling in Xenopus laevis oocytes and embryos, we have undertaken the characterization of the adapter protein Shc and studied its implication in oocyte maturation induced after IGF‐1 receptor activation, especially since expression of this molecule has been indirectly evidenced in Xenopus oocytes, eggs and embryos. We report herein the cloning from Xenopus postvitellogenic oocytes of a complementary DNA encoding a protein of 470 amino acids which shows the higher identity with the mammalian adaptor protein p52 ShcA . Western blot analysis using homologous antibodies evidenced a 60‐kDa protein, p60 Xl Shc , that is predominantly expressed in oocytes and in early embryos. We also demonstrate that, like p60 Xl Shc , Grb2 and the guanine nucleotide exchange factor Sos are expressed in oocytes throughout vitellogenesis and in early embryos and that overexpression of a dominant‐negative form of Grb2 specifically inhibits insulin‐induced resumption of meiosis. We finally show that Grb2 binds to p60 Shc in oocytes specifically upon insulin treatment. Altogether, these results suggest that Shc and Grb2‐Sos are implicated in ras‐dependent Xenopus oocyte maturation induced by insulin/IGF‐1; they also indicate that inability of insulin/IGF‐1 to activate the Ras‐MAPK cascade in vitellogenic oocytes does not result from an insufficient expression level of Shc, Grb2 and Sos.