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Primary response of naive CD4 + T cells to amino acid‐substituted analogs of an antigenic peptide can show distinct activation patterns: Th1‐ and Th2‐type cytokine secretion, and helper activity for antibody production without apparent cytokine secretion
Author(s) -
Ise Wataru,
Totsuka Mamoru,
Takato Rumi,
Hachimura Satoshi,
Sato Takehito,
Ametani Akio,
Kumagai Yoshihiro,
Habu Sonoko,
Kaminogawa Shuichi
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01716-0
Subject(s) - biology , cytokine , cytotoxic t cell , secretion , t cell , interleukin 21 , t helper cell , antigen , ovalbumin , antigen presenting cell , interleukin 4 , t cell receptor , immune system , microbiology and biotechnology , immunology , endocrinology , biochemistry , in vitro
Naive CD4 + T cells differentiate into two types of helper T cells showing an interferon‐γ‐predominant (Th1) or an interleukin‐4‐predominant (Th2) cytokine secretion profile after repeated antigenic stimulation. Their differentiation can be influenced by slight differences in the interaction between the T cell receptor (TCR) and its ligand at the time of primary activation. However, the primary response of freshly isolated naive CD4 + T cells to altered TCR ligands is still unclear. Here, we investigated the primary response of splenic naive CD4 + T cells derived from transgenic mice expressing TCR specific for residues 323–339 of ovalbumin (OVA323–339) bound to I‐A d molecules. Naive CD4 + T cells secreted either Th1‐ or Th2‐type cytokines immediately after stimulation with OVA323–339 or its single amino acid‐substituted analogs. Helper activity for antibody secretion by co‐cultured resting B cells was also found in the primary response, accompanied by either low‐level Th2‐type cytokine secretion or no apparent cytokine secretion. Our results clearly indicate that dichotomy of the Th1/Th2 cytokine secretion profile can be elicited upon primary activation of naive CD4 + T cells. We also demonstrate that the helper activity of naive CD4 + T cells for antibody production does not correspond to the amounts of the relevant cytokines secreted.