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Inhibition of MAP kinase blocks insulin‐mediated DNA synthesis and transcriptional activation of c‐ fos by Elk‐1 in vascular smooth muscle cells
Author(s) -
Xi Xiao-Ping,
Graf Kristof,
Goetze Stephan,
Hsueh Willa A,
Law Ronald E
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01303-3
Subject(s) - vascular smooth muscle , mapk/erk pathway , c fos , insulin , phosphorylation , dna synthesis , microbiology and biotechnology , kinase , mitogen activated protein kinase , transcription factor , transcription (linguistics) , chemistry , biology , smooth muscle , endocrinology , gene expression , dna , gene , biochemistry , linguistics , philosophy
Insulin‐stimulated DNA synthesis, MAP kinase (MAPK) activity and c‐ fos expression in vascular smooth muscle cells (VSMCs) was blocked by the MAPK inhibitor PD 98059. Regulation of c‐ fos expression by the transcription factor Elk‐1 at the serum response element (SRE) is dependent on its phosphorylation by MAPK. PD 98059 also suppressed insulin‐induced Elk‐1 transcriptional activity through the SRE. These data show that MAPK plays a critical role in both insulin‐mediated growth and Elk‐1‐dependent induction of c‐ fos in VSMCs.