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Mutational analysis of Yap1 protein, an AP‐1‐like transcriptional activator of Saccharomyces cerevisiae
Author(s) -
Takeuchi Tomonori,
Miyahara Kohji,
Hirata Dai,
Miyakawa Tokichi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01233-7
Subject(s) - yap1 , mutant , saccharomyces cerevisiae , mutagenesis , point mutation , microbiology and biotechnology , gene , biology , activator (genetics) , genetics , transcription factor
To define the essential amino acid residues of Yap1 in stress response, we generated yap1 mutations by in vitro mutagenesis, which cause defects in mediating resistance to the stress of H 2 O 2 , but not of CdCl 2 . Sequence analysis of the mutant yap1 genes revealed three point mutations and two truncation mutations near the carboxy‐terminus. The truncation mutations resulted in hyperresistance to cadmium. Northern blot analysis of stress‐induced levels of TRX2 and GSH1 mRNAs indicated that the ability of the mutant Yap1 protein to induce transcriptional activation of target genes correlates well with its ability to confer stress resistance. The carboxy‐terminal domain of Yap1 appears to act negatively in cadmium resistance.