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Transcriptional regulation of the Ras‐related protein TC21/R‐Ras2 in endothelial cells
Author(s) -
Detlef H. Kozian,
Hellmut G. Augustin
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01038-7
Subject(s) - autocrine signalling , northern blot , biology , signal transduction , microbiology and biotechnology , paracrine signalling , messenger rna , western blot , downregulation and upregulation , gene , cell culture , biochemistry , receptor , genetics
Applying an RNA display strategy to identify genes of autocrine activated endothelial cells, we identified among others the Ras‐related protein TC21/R‐Ras2 as a differentially expressed gene of bovine aortic endothelial cells (BAEC). Migrating BAEC express prominently upregulated steady state levels of TC21/R‐Ras2 mRNA (Northern blot, in situ hybridization) and protein (Western blot). Growth factor stimulation identified TC21/R‐Ras2 as aFGF, bFGF, and EGF inducible molecule of BAEC. Exposure to actinomycin D revealed a half life time of TC21/R‐Ras2 mRNA of 2h. These results strongly suggest that transcriptional regulation of Ras molecules contributes to their signal transduction capacity and a possible role of TC21/R‐Ras2 in the signal transduction of autocrine activated endothelial cells.