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Activation of mitogen‐activated protein kinase by the nociceptin receptor expressed in Chinese hamster ovary cells
Author(s) -
Fukuda Kazuhiko,
Shoda Takehiro,
Morikawa Hitoshi,
Kato Shigehisa,
Mori Kenjiro
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00815-6
Subject(s) - nociceptin receptor , chinese hamster ovary cell , mitogen activated protein kinase , protein kinase a , microbiology and biotechnology , receptor , chemistry , kinase , biology , biochemistry , opioid , opioid peptide
Activation of the nociceptin receptor stably expressed in Chinese hamster ovary cells induced a transient mitogen‐activated protein kinase (MAPK) activation, via pertussis toxin‐sensitive G‐proteins. The nociceptin receptor‐mediated MAPK activation was partially blocked by down‐regulation or inhibition of protein kinase C, and suppressed by pretreatment with a phosphatidylcholine‐specific phospholipase C inhibitor, D609. Furthermore, a tyrosine protein kinase inhibitor, genistein, and phosphatidylinositol 3‐kinase inhibitors, wortmannin and LY294002, affected the nociceptin‐induced MAPK activity. The nociceptin‐induced MAPK activation may lead to activation of phospholipase A 2 and induce changes in gene expression.