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The S.//.A.IG amino acid motif is present in a replication dependent late H3 histone variant of P. lividus sea urchin 1
Author(s) -
Fucci Laura,
Forte Amalia,
Mancini Pietro,
Affaitati Adele,
Branno Margherita,
Aniello Francesco,
Geraci Giuseppe
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00305-0
Subject(s) - biology , histone , histone h3 , microbiology and biotechnology , paracentrotus lividus , gene , genetics , sea urchin
A novel gene encoding a new H3 histone variant (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3′ terminal stem‐loop nucleotide sequence that is typical of the replication dependent variants. The gene is intronless, the corresponding short transcript is non‐polyadenylated and its expression is replication dependent with a timing of a late variant. The new H3 variant is expressed as a minor component with respect to a major replication dependent late H3 histone here identified by partial cDNA sequence. These results show that classification of histones in replication dependent and independent variants only on the basis of their amino acid sequences should be reconsidered.