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Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization
Author(s) -
Marin Silvia,
Chiang Kelly,
Bassilian Sara,
Lee Wai-Nang Paul,
Boros Laszlo G,
Fernández-Novell Josep Maria,
Centelles Josep Joan,
Medrano Antonio,
Rodriguez-Gil Joan Enric,
Cascante Marta
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01185-2
Subject(s) - gluconeogenesis , boar , citric acid cycle , biochemistry , glycolysis , metabolic pathway , glycogen , metabolomics , chemistry , pyruvic acid , biology , lactate dehydrogenase , sperm , metabolism , enzyme , chromatography , genetics
Metabolomic characteristics in boar spermatozoa were studied using [1,2‐ 13 C 2 ]glucose and mass isotopomer analysis. In boar spermatozoa, glycolysis was the main pathway of glucose utilization producing lactate/pyruvate, whereas no gluconeogenesis was seen. Slight glycogen synthesis through the direct pathway and some incorporation of pyruvate into the Krebs cycle also took place. Neither RNA ribose‐5‐phosphate nor fatty acid synthesis from glucose occurred despite the detection of pyruvate dehydrogenase activity. In contrast to the known metabolic activities in dog sperm, boar spermatozoa have low levels of energy production and biosynthetic activities suggesting two different metabolic profiles for the two different phenotypes.