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Oxidative DNA damage by hyperglycemia‐related aldehydes and its marked enhancement by hydrogen peroxide
Author(s) -
Murata Mariko,
Mizutani Mika,
Oikawa Shinji,
Hiraku Yusuke,
Kawanishi Shosuke
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01129-3
Subject(s) - dna damage , 8 hydroxy 2' deoxyguanosine , hydrogen peroxide , deoxyguanosine , chemistry , dna , dna oxidation , oxidative damage , oxidative phosphorylation , endogeny , oxidative stress , biochemistry , diabetes mellitus , carcinogenesis , catalase , endocrinology , biology , gene
Increased risks of cancers and oxidative DNA damage have been observed in diabetic patients. Many endogenous aldehydes such as 3‐deoxyglucosone and glyceraldehyde (GA) increase under hyperglycemic conditions. We showed that these aldehydes induced Cu(II)‐mediated DNA damage, including 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine (8‐oxodG) formation. GA had the strongest ability to damage DNA, and addition of low concentrations of H 2 O 2 markedly enhanced the DNA damage. GA significantly increased 8‐oxodG formation in human cultured cells (HL‐60), and H 2 O 2 enhanced it. We conclude that oxidative DNA damage by hyperglycemia‐related aldehydes, especially GA, and marked enhancement of DNA damage by H 2 O 2 may participate in diabetes‐associated carcinogenesis.