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Specific covalent binding of a NF‐κB decoy hairpin oligonucleotide targeted to the p50 subunit and induction of apoptosis
Author(s) -
Lesage Denis,
Metelev Valeri,
Borisova Oksana,
Dolinnaya Nina,
Oretskaya Tatiana,
Baran-Marszak Fanny,
Taillandier Eliane,
Raphael Martine,
Fagard Remi
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00689-6
Subject(s) - decoy , p50 , oligonucleotide , protein subunit , apoptosis , cell growth , microbiology and biotechnology , nf κb , immune system , chemistry , cell culture , biology , cancer research , transcription factor , dna , biochemistry , receptor , immunology , gene , genetics
The NF‐κB transcriptional factor regulates various functions such as immune responses, cellular growth and development, and is frequently activated in tumor cells. Thus, inhibition of NF‐κB could suppress tumor cell growth. Using a decoy synthetic hairpin‐shaped oligodeoxyribonucleotide (ODN) containing the κB site with an integrated single diphosphoryldisulfide linkage, we demonstrate its covalent binding to the p50 subunit of NF‐κB. Furthermore, this decoy ODN induces apoptosis in a lymphoblastoma cell line. Thus, such chemically modified decoys could be valuable tools for blocking nuclear factors and tumor cell growth.