Specific conjugation of DNA binding proteins to DNA templates through thiol–disulfide exchange | Zendy

Metelev Valeri G. | Zendy; Kubareva Elena A. | Zendy; Vorob'eva Olga V. | Zendy; Romanenkov Andrey S. | Zendy; Oretskaya Tatiana S. | Zendy

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Specific conjugation of DNA binding proteins to DNA templates through thiol–disulfide exchange

Author(s) -

Metelev Valeri G.,

Kubareva Elena A.,

Vorob'eva Olga V.,

Romanenkov Andrey S.,

Oretskaya Tatiana S.

Publication year - 2003

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/s0014-5793(03)00122-4

Subject(s) - template , dna , cysteine , chemistry , covalent bond , thiol , disulfide bond , combinatorial chemistry , sequence (biology) , biochemistry , enzyme , nanotechnology , organic chemistry , materials science

The double‐stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase Sso II to sequence‐specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.

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