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Autophagy and the cytoplasm to vacuole targeting pathway both require Aut10p
Author(s) -
Barth Henning,
Meiling-Wesse Khuyen,
Epple Ulrike D,
Thumm Michael
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03016-2
Subject(s) - autophagy , vacuole , cytoplasm , microbiology and biotechnology , cytosol , biology , endogeny , bag3 , immunofluorescence , chemistry , biochemistry , genetics , enzyme , apoptosis , antibody
We here report the identification of AUT10 as a novel gene required for both the cytoplasm to vacuole targeting of proaminopeptidase I and starvation‐induced autophagy. aut10 Δ cells are impaired in maturation of proaminopeptidase I under starvation and non‐starvation conditions. A lack of Aut10p causes a defect in autophagy prior to vacuolar uptake of autophagosomes. Homozygous aut10 Δ diploids do not sporulate. Vacuolar acidification indicated by accumulation of quinacrine is normal in aut10 Δ cells and mature vacuolar proteinases are present. A biologically active Ha‐tagged Aut10p, chromosomally expressed from its endogenous promoter, localizes in indirect immunofluorescence microscopy in the cytosol and on granulated structures, which appear clustered around the vacuolar membrane. This localization differs from known autophagy proteins.