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Differential inheritance modes of DNA methylation between euchromatic and heterochromatic DNA sequences in ageing fetal bovine fibroblasts
Author(s) -
Kang Yong-Kook,
Koo Deog-Bon,
Park Jung Sun,
Choi Young-Hee,
Lee Kyung-Kwang,
Han Yong-Mahn
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02472-3
Subject(s) - biology , euchromatin , dna methylation , heterochromatin , microbiology and biotechnology , genetics , bisulfite sequencing , epigenomics , epigenetics , methylation , dna , gene , chromosome , gene expression
To elucidate overall changes in DNA methylation occurring by inappropriate epigenetic control during ageing, we compared fetal bovine fibroblasts and their aged neomycin‐resistant versions using bisulfite–PCR technology. Reduction in DNA methylation was observed in euchromatic repeats (18S‐ rRNA / art2 ) and promoter regions of single‐copy genes (the cytokeratin/β‐lactoglobulin/interleukin‐13 genes). Contrastingly, a stable maintenance of DNA methylation was revealed in various heterochromatic sequences (satellite I/II/alphoid and Bov‐B ). The differential inheritance mode of DNA methylation was confirmed through the analysis of individual neomycin‐resistant clones. These global, multi‐locus analyses provide evidence on the tendency of differential epigenetic modification between genomic DNA regions during ageing.