Perinuclear mRNA localisation by vimentin 3′‐untranslated region requires a 100 nucleotide sequence and intermediate filaments | Zendy

Bermano Giovanna | Zendy; Shepherd Rebecca K. | Zendy; Zehner Zendra E. | Zendy; Hesketh John E. | Zendy

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Perinuclear mRNA localisation by vimentin 3′‐untranslated region requires a 100 nucleotide sequence and intermediate filaments

Author(s) -

Bermano Giovanna,

Shepherd Rebecca K.,

Zehner Zendra E.,

Hesketh John E.

Publication year - 2001

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/s0014-5793(01)02438-3

Subject(s) - vimentin , intermediate filament , messenger rna , untranslated region , three prime untranslated region , microbiology and biotechnology , cytoplasm , transfection , biology , nucleic acid sequence , cell culture , gene , cell , cytoskeleton , biochemistry , genetics , immunohistochemistry , immunology

The role of the vimentin 3′‐untranslated region (3′‐UTR) in mRNA localisation was studied in cells transfected with a reporter sequence linked to subregions of the 3′‐UTR. In situ hybridisation showed that nucleotides 37–137, including a previously identified protein‐binding domain, were sufficient to localise transcripts to perinuclear cytoplasm. Transfection of two SW13 cell lines that do and do not express vimentin showed that perinuclear localisation due to either the vimentin or c‐myc 3′‐UTR requires intermediate filaments. The data suggest that both a specific protein‐binding region of the vimentin 3′‐UTR and intermediate filaments themselves are required to determine the site of vimentin synthesis.

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