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ESR studies on reaction of saccharide with the free radicals generated from the xanthine oxidase/hypoxanthine system containing iron
Author(s) -
Luo Gui-min,
Qi Dan-hua,
Zheng Ying-guang,
Mu Ying,
Yan Gang-ling,
Yang Tong-shu,
Shen Jia-Cong
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02226-8
Subject(s) - chemistry , hypoxanthine , radical , xanthine oxidase , superoxide , xanthine , molecule , spin trapping , hydroxyl radical , medicinal chemistry , stereochemistry , organic chemistry , enzyme
The free radicals generated from the iron containing system of xanthine oxidase and hypoxanthine (Fe‐XO/HX) were directly detected by using spin trapping. It was found that not only superoxide anion (O 2 − ) and hydroxyl radical (OH), but also alkyl or alkoxyl radicals (R) were formed when saccharides such as glucose, fructose and sucrose were added into the Fe‐XO/HX system. The generated amount of R was dependent on the kind and concentration of saccharides added into the Fe‐XO/HX system and no R were detected in the absence of saccharides, indicating that there is an interaction between the saccharide molecules and the free radicals generated from the Fe‐XO/HX system and saccharide molecules are essential for generating R in the Fe‐XO/HX system. It is expected that the toxicity of R would be greater than of hydrophilic O 2 − and OH because they are liposoluble and their lives are longer and the active sites of biomolecules are closely related with lipophilic phase, thus they can damage cells more seriously than O 2 − and OH. The R generated from the saccharide containing Fe‐XO/HX can be effectively scavenged by selenium containing abzyme (Se‐abzyme), indicating Se‐abzyme is a promising antioxidant.