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Inhibition of influenza virus replication in cultured cells by RNA‐cleaving DNA enzyme
Author(s) -
Toyoda Tetsuya,
Imamura Yoshihiro,
Takaku Hiroshi,
Kashiwagi Takahito,
Hara Koyu,
Iwahashi Jun,
Ohtsu Yasushi,
Tsumura Naoki,
Kato Hirohisa,
Hamada Nobuyuki
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01974-8
Subject(s) - rna , oligonucleotide , enzyme , biology , dna , microbiology and biotechnology , viral replication , antisense rna , sense (electronics) , influenza a virus , virus , virology , messenger rna , in vitro , gene , chemistry , biochemistry
Influenza virus replication has been effectively inhibited by antisense phosphothioate oligonucleotides targeting the AUG initiation codon of PB2 mRNA. We designed RNA‐cleaving DNA enzymes from 10‐23 catalytic motif to target PB2‐AUG initiation codon and measured their RNA‐cleaving activity in vitro. Although the RNA‐cleaving activity was not optimal under physiological conditions, DNA enzymes inhibited viral replication in cultured cells more effectively than antisense phosphothioate oligonucleotides. Our data indicated that DNA enzymes could be useful for the control of viral infection.