Premium
Involvement of an FtsH homologue in the assembly of functional photosystem I in the cyanobacterium Synechocystis sp. PCC 6803
Author(s) -
Mann Nicholas H,
Novac Natalia,
Mullineaux Conrad W,
Newman Julie,
Bailey Shaun,
Robinson Colin
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01871-8
Subject(s) - phycobilisome , synechocystis , photosystem ii , biogenesis , cyanobacteria , photosystem , photosystem i , chemistry , mutagenesis , biochemistry , biology , mutant , biophysics , photosynthesis , gene , genetics , bacteria
The Synechocystis sp. PCC 6803 genome encodes four putative homologues of the AAA protease FtsH, two of which (slr0228 and sll1463) have been subjected to insertional mutagenesis in this study. Disruption of sll1463 had no discernible effect but disruption of slr0228 caused a 60% reduction in the abundance of functional photosystem I, without affecting the cellular content of photosystem II or phycobilisomes. Fluorescence and immunoblotting analyses show reductions in PS I polypeptides and possible structural alterations in the residual PS I, indicating an important role for slr0228 in PS I biogenesis.