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Functional Fv fragment of an antibody specific for CD28: Fv‐mediated co‐stimulation of T cells
Author(s) -
Takemura Shin-ichi,
Asano Ryutaro,
Tsumoto Kouhei,
Arai Takashi,
Sakurai Naoki,
Kodama Hideaki,
Yoshida Hiroshi,
Katayose Yu,
Suzuki Masanori,
Matsuno Seiki,
Kudo Toshio,
Kumagai Izumi
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01741-5
Subject(s) - cd28 , microbiology and biotechnology , monoclonal antibody , antibody , biology , co stimulation , t cell , chemistry , immune system , immunology
The most predominant co‐stimulation pathway, which is critical for T cell activation and proliferation, is the CD28‐B7 pathway. The anti‐CD28 monoclonal antibody (mAb) also provides a co‐stimulatory signal to T cells. In order to construct a functional Fv fragment (complex of VH and VL domains) of anti‐CD28 antibody using a bacterial expression system, cDNA encoding the variable regions of immunoglobulin from 15E8 hybridoma cells was cloned and expressed in Escherichia coli . The Fv fragment was obtained as a soluble protein from the periplasmic fraction and showed a binding pattern similar to parental IgG. The Fv fragment induced proliferation of peripheral blood mononuclear cells in the presence of anti‐CD3 or anti‐CD2 mAb and enhanced anti‐tumor activity of anti‐MUC1×anti‐CD3 bispecific antibody when tested with lymphokine‐activated killer cells with T cell phenotype. Thus, the anti‐CD28 Fv fragment will be promising not only for the study of co‐stimulation, but also for cancer immunotherapy.