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Secondary structure of the 5′‐region of PGY1/MDR1 mRNA
Author(s) -
Kostenko Elena V.,
Beabealashvilly Robert Sh.,
Vlassov Valentin V.,
Zenkova Marina A.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01650-1
Subject(s) - messenger rna , oligonucleotide , protein secondary structure , nucleotide , microbiology and biotechnology , chemistry , biology , gene , biochemistry
In order to identify the optimal target sites for antisense oligonucleotides in the human multiple drug resistance mRNA, the secondary structure of the 5′‐terminal part of this mRNA (nucleotides 1–678) was investigated. By using results of probing with ribonucleases T1, ONE and V1 and results of computer simulations, a model of the 5′‐region of the PGY1/MDR1 mRNA was built. The molecule is formed by three major domains comprising several hairpins separated by single‐stranded fragments. The predicted single‐stranded regions of the PGY1/MDR1 mRNA efficiently bind complementary oligonucleotides.