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Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli
Author(s) -
Rodrı́guez-Concepción Manuel,
Campos Narciso,
Marı́a Lois Luisa,
Maldonado Carlos,
Hoeffler Jean-François,
Grosdemange-Billiard Catherine,
Rohmer Michel,
Boronat Albert
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01552-0
Subject(s) - escherichia coli , biochemistry , terpenoid , plastid , biosynthesis , isomerase , mevalonate pathway , farnesyl diphosphate synthase , branching (polymer chemistry) , chemistry , gene , biology , organic chemistry , chloroplast
An alternative mevalonate‐independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli ) and plant plastids, although it is not fully elucidated yet. In this work, E. coli cells were engineered to utilize exogenously provided mevalonate and used to demonstrate by a genetic approach that branching of the endogenous pathway results in separate synthesis of the isoprenoid building units isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In addition, the IPP isomerase encoded by the idi gene was shown to be functional in vivo and to represent the only possibility for interconverting IPP and DMAPP in this bacterium.