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P1‐137: Transthyretin in Aβ clearance: Establishment of choroid plexus cell lines
Author(s) -
Oliveira Sandra Marisa,
Alves Isabel Longo,
Trigo Diogo,
João Saraiva Maria
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.724
Subject(s) - choroid plexus , transthyretin , lrp1 , neuroprotection , endocytic cycle , cerebrospinal fluid , blood–brain barrier , microbiology and biotechnology , chemistry , transfection , receptor , cell culture , biology , neuroscience , endocrinology , endocytosis , central nervous system , biochemistry , ldl receptor , lipoprotein , genetics , cholesterol
populations by immunocytochemistry, Western Blot and QPCR analysis. Results: We determined the changes in Notch pathway, as well as the neural differentiation stages. Transient treatment on hESC cultures inhibited the neural induction, but on hESC-derived neural progenitor stages caused a massive and coordinated differentiation of neural progenitors into neuronal populations. Conclusions: Further work is needed in order to characterize the effect of gamma-secretase inhibitors in combination with different patterning strategies. Defining the in vitro effects of gamma secretase inhibitors on different human telencephalic populations will help in reducing the side-effects of these potential drugs for AD treatment.