Premium
P1‐444: Increased formation of amyloid‐β peptide in CHO NPC1‐null cells does not involve lipid rafts
Author(s) -
Kosicek Marko,
Goate Alison,
Hecimovic Silva
Publication year - 2008
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2008.05.1026
Subject(s) - lipid raft , cholesterol , npc1 , raft , chemistry , biochemistry , transfection , microbiology and biotechnology , biology , intracellular , endosome , organic chemistry , copolymer , polymer , gene
cells. When cholesterol in CHOwt cells was raised to the levels seen in M12 cells, the A levels were still significantly higher in M12 cells than in CHOwt. Conclusions: Our results suggest that increased levels of A upon cholesterol accumulation in M12 cells are not directly related to cholesterol levels. We assume that the differences in A levels in M12 cells may be due to disturbed endosoma/lysosomal trafficking in these cells. This work was funded by the grants: NIH-FIRCA 1R03TW007335-01 (to A.G.) and Ministry of Science of the Republic of Croatia 098-0982522-2525 (to S.H.).