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Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae : Importance in normal cell growth, stress resistance, and longevity
Author(s) -
Sakurai Hiroshi,
Ota Azumi
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.07.041
Subject(s) - heat shock factor , biology , promoter , saccharomyces cerevisiae , transcription factor , heat shock protein , microbiology and biotechnology , gene , transcription (linguistics) , gene expression , hsf1 , chaperone (clinical) , hsp90 , regulation of gene expression , heat shock , genetics , hsp70 , medicine , linguistics , philosophy , pathology
Heat shock transcription factor (HSF), a key regulator in the expression of heat shock protein (HSP) chaperones, is involved in the maintenance of protein homeostasis. However, the impact of HSF‐mediated transcription of each HSP gene on this process is not fully understood. We show that Saccharomyces cerevisiae cells containing mutations in the HSF‐binding sequences of chromosomal HSP90 promoters exhibit various phenotypes, including slow growth, proteotoxic stress sensitivity, and reduced chronological lifespan. Similar phenotypes were observed when HSF‐binding sequences in five mitochondrial HSP promoters were mutated. Therefore, HSF‐regulated changes in expression of these chaperone genes are necessary to maintain cell viability under various growth conditions.