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Arabidopsis actin‐depolymerizing factors (ADFs) 1 and 9 display antagonist activities
Author(s) -
Tholl Stéphane,
Moreau Flora,
Hoffmann Céline,
Arumugam Karthik,
Dieterle Monika,
Moes Danièle,
Neumann Katrin,
Steinmetz André,
Thomas Clément
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.05.019
Subject(s) - actin , actin remodeling , arabidopsis , depolymerization , microbiology and biotechnology , actin binding protein , cytoskeleton , actina , in vitro , actin cytoskeleton , mdia1 , antagonist , biology , microfilament , chemistry , biochemistry , cell , gene , receptor , organic chemistry , mutant
We provide evidence that one of the 11 Arabidopsis actin‐depolymerizing factors (ADFs), namely ADF9, does not display typical F‐actin depolymerizing activity. Instead, ADF9 effectively stabilizes actin filaments in vitro and concomitantly bundles actin filaments with the highest efficiency under acidic conditions. Competition experiments show that ADF9 antagonizes ADF1 activity by reducing its ability to potentiate F‐actin depolymerization. Accordingly, ectopic expression of ADF1 and ADF9 in tobacco cells has opposite effects. ADF1 severs actin filaments/bundles and promotes actin cytoskeleton disassembly, whereas ADF9 induces the formation of long bundles. Together these data reveal an additional degree of complexity in the comprehension of the biological functions of the ADF family and illustrate that antagonist activities can be displayed by seemingly equivalent actin‐binding proteins.
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