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Revival of apoptotic cells that display early‐stage dynamic membrane blebbing
Author(s) -
Takashina Tomoki,
Nakayama Manabu
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.033
Subject(s) - apoptosis , fadd , microbiology and biotechnology , programmed cell death , effector , caspase , biology , chemistry , biochemistry
The critical point at which apoptosis becomes irreversible and how cells attain an anti‐apoptotic state remain unknown. Here, we report that apoptotic cells undergoing early‐stage dynamic membrane blebbing revive. We examined this phenomenon in cell lines that stably express 2DED2DD, a modified FADD produced by fusing the tandem death effector domains (DEDs) and tandem death domains (DDs). Induction of apoptosis caused rapid blebbing. Eight hours later, most cells shrunk while some detached from the flask. Twenty‐four hours later, when activated caspase 3 decreased, more than half the cells revived and appeared normal, probably due to the induction of unidentified anti‐apoptotic proteins.