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In vitro and in vivo studies on CCR10 regulation by Annexin A1
Author(s) -
Rodrigues-Lisoni Flavia Cristina,
Mehemet Devinder K.,
Peitl Paulo,
John Christopher D.,
Tajara Eloiza,
Buckingham Julia C.,
Solito Egle
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.01.072
Subject(s) - annexin a1 , c c chemokine receptor type 6 , chemokine receptor , downregulation and upregulation , microbiology and biotechnology , chemokine , receptor , biology , gene , cancer research , annexin , chemistry , flow cytometry , biochemistry
The mode of action of annexin A1 (ANXA1) is poorly understood. By using rapid subtraction hybridization we studied the effects of human recombinant ANXA1 and the N‐terminal ANXA1 peptide on gene expression in a human larynx cell line. Three genes showed strong downregulation after treatment with ANXA1. In contrast, expression of CCR10 , a seven transmembrane G‐protein coupled receptor for chemokine CCL27 involved in mucosal immunity, was increased. Moreover the reduction in CCR10 expression induced by ANXA1 gene deletion was rescued by intravenous treatment with low doses of ANXA1. These findings provide new evidence that ANXA1 modulates gene expression.