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Cathepsin B and its interacting proteins, bikunin and TSRC1, correlate with TNF‐induced apoptosis of ovarian cancer cells OV‐90
Author(s) -
Liu Jianping,
Guo Qian,
Chen Buxin,
Yu Yao,
Lu Hong,
Li Yu-Yang
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.12.005
Subject(s) - apoptosis , tumor necrosis factor alpha , cathepsin d , cancer , ovarian cancer , cancer research , chemistry , microbiology and biotechnology , medicine , endocrinology , biology , enzyme , biochemistry
Increasing evidence suggests that lysosomal cysteine proteases cathepsins contribute to the progression of cell apoptosis. Here we found that apoptosis of ovarian cancer cells OV‐90 triggered by TNF was cathepsin B‐depended. Two cathepsin B binding proteins, bikunin and TSRC1, were identified by yeast two‐hybrid method and the interactions were confirmed in vitro and in vivo. Overexpression of bikunin could suppress TNF‐induced apoptosis of OV‐90 cells, and TSRC1 overexpression had an opposite effect on apoptosis. The presented results suggest that cathepsin B and its interacting proteins, bikunin and TSRC1, are involved in the apoptotic pathway of ovarian cancer cells.