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Identification and characterization of small RNAs involved in RNA silencing
Author(s) -
Aravin Alexei,
Tuschl Thomas
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.08.009
Subject(s) - rna silencing , trans acting sirna , rna induced silencing complex , gene silencing , rna , rna interference , biology , rna induced transcriptional silencing , dna directed rna interference , small nucleolar rna , argonaute , computational biology , non coding rna , genetics , gene
Double‐stranded RNA (dsRNA) is a potent trigger of sequence‐specific gene silencing mechanisms known as RNA silencing or RNA interference. The recognition of the target sequences is mediated by ribonucleoprotein complexes that contain 21‐ to 28‐nucleotide (nt) guide RNAs derived from processing of the trigger dsRNA. Here, we review the experimental and bioinformatic approaches that were used to identify and characterize these small RNAs isolated from cells and tissues. The identification and characterization of small RNAs and their expression patterns is important for elucidating gene regulatory networks.