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A fourth ADP/ATP carrier isoform in man: identification, bacterial expression, functional characterization and tissue distribution
Author(s) -
Dolce Vincenza,
Scarcia Pasquale,
Iacopetta Domenico,
Palmieri Ferdinando
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.12.034
Subject(s) - atp–adp translocase , subfamily , mitochondrion , gene isoform , biochemistry , mitochondrial matrix , cytosol , biology , adenosine triphosphate , microbiology and biotechnology , heterologous expression , inner mitochondrial membrane , enzyme , chemistry , gene , recombinant dna
The mitochondrial ADP/ATP carriers (AACs) catalyze the exchange of cytosolic ADP for matrix ATP. We have identified and characterized a novel member of the AAC subfamily of mitochondrial metabolite transport proteins, termed AAC4. The AAC4 gene maps to human chromosome 4q28.1, and its product AAC4 is 66–68% identical to human AAC 1–3 and is localized to mitochondria. AAC4 transcripts are exclusively present in liver, testis and brain unlike those of AAC 1–3. Consistent with its belonging to the AAC subfamily, upon heterologous expression and reconstitution into liposomes AAC4 exchanges ADP for ATP by an electrogenic antiport mechanism with high specificity and high sensitivity to carboxyatractyloside and bongkrekic acid.