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Enhancement of the polycation‐mediated DNA uptake and cell transfection with Pluronic P85 block copolymer
Author(s) -
Astafieva Irina,
Maksimova Irina,
Lukanidin Eugenii,
Alakhov Valery,
Kabanov Alexander
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00607-2
Subject(s) - poloxamer , copolymer , transfection , chemistry , dna , biophysics , microbiology and biotechnology , biochemistry , gene , biology , polymer , organic chemistry
Polyelectrolyte complexes formed between DNA and poly( N ‐ethyl‐4‐vinylpyridinium) cations were shown to effectively transfect mammalian cells [7]. This work suggests that the polycation‐mediated uptake of the plasmid DNA and cell transfection are significantly enhanced when these complexes are administered simultaneously with a poly(ethylene oxide) block ‐poly(propylene oxide)‐ block ‐poly(ethylene oxide) copolymer, Pluronic P85. The uptake studies were performed using radioactively labeled pRSV CAT plasmid on NIH 3T3, MDCK, and Jurkat cell lines. The transfection was investigated by chloramphenicol acetyltransferase assay using 3T3 cells as a model. The effects reported may be useful for the enhancement of the polycation‐mediated cell transfection.