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The third intracellular domain of the m3 muscarinic receptor determines coupling to calcium influx in transfected Chinese hamster ovary cells
Author(s) -
Singer-Lahat Dafna,
Liu Jie,
Wess Jurgen,
Felder Christian C.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00398-5
Subject(s) - chinese hamster ovary cell , muscarinic acetylcholine receptor , calcium in biology , microbiology and biotechnology , receptor , muscarinic acetylcholine receptor m3 , intracellular , muscarinic acetylcholine receptor m5 , muscarinic acetylcholine receptor m2 , calcium , muscarinic acetylcholine receptor m1 , chemistry , transfection , biology , biophysics , biochemistry , organic chemistry , gene
The m2 and m3 muscarinic acetylcholine receptors were expressed in CHO cells and were shown to couple to the release of calcium from intracellular stores. The m3 receptor, but not the m2 receptor, also coupled to calcium influx. Chimeric m2/m3 receptors were used to determine the structural domain of the m3 receptor linked to the regulation of calcium influx. It was found that the third intracellular loop of m3 receptor plays a fundamental role in regulating Ca 2+ influx predicted to occur through Ca 2+ channels located in the plasma membrane in CHO cells.