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cDNA analysis of the mite allergen Lep d 1 identifies two different isoallergens and variants
Author(s) -
Schmidt Margit,
Olsson Susanna,
van der Ploeg Ingeborg,
van Hage-Hamsten Marianne
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)98164-e
Subject(s) - complementary dna , heteroduplex , amino acid , microbiology and biotechnology , biology , allergen , oligonucleotide , peptide sequence , genetics , gene , immunology , allergy
For the first time the complete cDNA encoding two isoallergens of the non‐pyroglyphid dust mite Lepidoglyphus destructor , Lep d 1, allergen has been sequenced. In addition, one of the isoallergens was found to have two variants. Oligonucleotides were designed from known amino acid sequences. The cDNA sequences were obtained by hybridizing these primers to mRNA and enhancement by the RT‐PCR technique. To obtain the different complete encoding cDNA sequences and eliminate heteroduplex artifacts, we performed PCR + 1 reactions. Comparison of the amino acid sequence of the allergen shows leader sequences of 16 amino acids for both isoforms.