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Tumor necrosis factor (TNF) stimulates the production of nerve growth factor in fibroblasts via the 55‐kDa type 1 TNF receptor
Author(s) -
Hattori Akira,
Hayashi Kyozo,
Kohno Michiaki
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01502-7
Subject(s) - tumor necrosis factor alpha , nerve growth factor , receptor , tumor necrosis factor receptor , chemistry , tumor necrosis factor α , necrosis , microbiology and biotechnology , endocrinology , medicine , cancer research , biology , biochemistry
The role of the two types of tumor necrosis factor (TNF) receptors, TNF‐RI and TNF‐R2, in mediating the capacity of TNF to stimulate nerve growth factor (NGF) production in fibroblasts has been investigated. Although Swiss 3T3 cells express both the TNF‐R1 and TNF‐R2, an agonistic anti‐TNF R1 antibody, but not an agonistic anti‐TNF‐R2 antibody, increases the NGF mRNA level and stimulates the production of NGF protein in the cells. Treatment of the cells with a combination of anti‐TNF‐R2 antibody and interleukin‐1β (IL‐1β) does not increase the NGF production induced by IL‐1β alone, although TNF's activity to stimulate NGF production is markedly enhanced by IL‐1β. Furthermore, simultaneous addition of the both antibodies does not increase the NGF production above that observed with the anti‐TNF‐RI antibody, indicating that TNF‐R1 alone mediates the TNF's activity to stimulate NGF production in fibroblasts.