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Molecular cloning of a human polypeptide related to yeast sds22, a regulator of protein phosphatase‐1
Author(s) -
Renouf Sylvie,
Beullens Monique,
Wera Stefaan,
Van Eynde Aleyde,
Sikela James,
Stalmans Willy,
Bollen Mathieu
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01180-m
Subject(s) - complementary dna , cytoplasm , yeast , cloning (programming) , molecular cloning , microbiology and biotechnology , biology , phosphatase , saccharomyces cerevisiae , biochemistry , two hybrid screening , molecular mass , nuclear protein , gene , enzyme , transcription factor , computer science , programming language
sds22 is a regulatory polypeptide of protein phosphatase‐1 that is required for the completion of mitosis in both fission and budding yeast. We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucinerich repeat structures of 22 residues. Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.