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Poliovirus 2A pro expression inhibits growth of yeast cells
Author(s) -
Barco Angel,
Carrasco Luis
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00841-v
Subject(s) - proteases , poliovirus , yeast , saccharomyces cerevisiae , cytoplasm , translation (biology) , microbiology and biotechnology , biology , protease , rna , protein biosynthesis , gene expression , messenger rna , virology , biochemistry , virus , gene , enzyme
Poliovirus encodes two proteases, 2A pro and 3C pro that participate in the processing of the viral polyprotein and cleave a number of host proteins. Both proteases have been cloned and expressed in an inducible manner in Saccharomyces cerevisiae cells. The expression of 2A pro , but not 3C pro , was highly toxic for yeast cells such that growth was arrested after 5 h of induction and cell survival sharply declined. Cellular morphology was profoundly modified by expression of poliovirus 2A pro , in such a way that electron dense granules and autophagosomic bodies arise in the cytoplasm. Experiments aimed at defining the yeast function affected by 2A pro suggested that translation was not the target of protease toxicity, but showed that RNA synthesis was profoundly blocked.