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Effects of protein kinase inhibitors and protein phosphatase inhibitors on cyclic AMP‐dependent down‐regulation of vesicular monoamine transport in pheochromocytoma PC12 cells
Author(s) -
Nobuo Nakanishi,
Satoshi Onozawa,
Reiko Matsumoto,
Kinji Kurihara,
T Ueha,
Hiroyuki Hasegawa,
Naomi Minami
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00698-9
Subject(s) - monoamine neurotransmitter , protein kinase a , okadaic acid , biogenic amine , phosphatase , vesicular transport protein , chemistry , phosphorylation , protein kinase inhibitor , vesicular monoamine transporter 2 , kinase , biochemistry , microbiology and biotechnology , biology , serotonin , vesicle , receptor , membrane
Cyclic AMP down‐regulates vesicular monoamine transport in PC12 cells and thereby decreased catecholamine reuptake from the extracellular fluid. We examined the effects of protein kinase inhibitors and protein phosphatase inhibitors on this cAMP action. Treatment of cells with a protein kinase inhibitor, K252a, increased vesicular amine transport and cellular amine uptake, thereby antagonizing the regulatory action of cAMP. In contrast, a protein phosphatase inhibitor, okadaic acid, had the opposite effect on the amine transport, i.e. it enhanced the cAMP action. These results suggest the involvement of a protein phosphorylation process in the cAMP‐dependent modulation of vesicular monoamine transport.