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Antisense retinoic acid receptor γ‐1 oligonucleotide enhances chondrogenesis of mouse limb mesenchymal cells in vitro
Author(s) -
Motoyama Jun,
Eto Kazuhiro
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80291-2
Subject(s) - chondrogenesis , retinoic acid , limb bud , mesenchymal stem cell , retinoic acid receptor , limb development , microbiology and biotechnology , in vitro , biology , chemistry , cellular differentiation , embryo , gene , biochemistry
Retinoic acid receptor (RAR) γ gene is expressed in the precartilaginous cells during chondrogenesis in mouse embryos, but the role of the gene products is still unclear. To examine the role during chondrogenesis, we isolated mesenchymal cells from the limb bud of mouse embryos and exposed them to antisense RAR γ‐1 oligodeoxynucleotide in micromass culture. The antisense oligodeoxynucleotide inhibited RAR γ‐1 protein expression and enhanced chondrogenesis in the exposed cells. These results suggest that the complex of RAR γ‐1 protein and its ligand RA acts as a suppressor of the chondrogenesis in the limb development.