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Molecular identification of catalases from Nicotiana plumbaginifolia (L.)
Author(s) -
Willekens Hilde,
Villarroel Raimundo,
Van Montagu Marc,
Inzé Dirk,
Van Camp Wim
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00923-6
Subject(s) - catalase , salicylic acid , paraquat , biochemistry , enzyme , microbiology and biotechnology , biology , messenger rna , chemistry , gene
We have isolated three different catalase cDNAs from Nicotiana plumbaginifolia ( cat1 , cat2 , and cat3 ) and a partial sequence of a fourth catalase gene ( cat4 ) that shows no discernible expression based on Northern analysis. The catalase sequences were used to determine the similarity with other plant catalases and to study the transcriptional response to paraquat, 3‐aminotriazole, and salicylic acid. 3‐Aminotriazole induces mRNA levels of cat1 , cat2 and cat3 , indicating that a reduction in catalase activity positively affects catalase mRNA abundance. Salicylic acid that binds catalase in vitro, had no effect on catalase transcript levels at physiological concentrations. Paraquat resulted in the induction of cat1 .