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Multiple nuclear localization signals of the B‐ myb gene product
Author(s) -
Takemoto Yoshihiro,
Tashiro Shigeki,
Handa Hiroshi,
Ishii Shunsuke
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00733-0
Subject(s) - nls , nuclear localization sequence , nuclear transport , nuclear protein , transcription factor , gene product , biology , myb , gene , microbiology and biotechnology , genetics , gene expression , cell nucleus
Nuclear entry of the B‐ myb gene product (B‐Myb) is dependent on multiple nuclear localization signals (NLS's). Mutagenesis of the putative NLS's of B‐Myb has identified two separate NLS's, NLS1 and NLS2. Each of the two NLS's is essential for efficient nuclear targeting. NLS2 contains two interdependent basic domains separated by 8 intervening spacer amino acids, and both basic domains are required for nuclear entry. Thus, NLS2 belongs to a class of bipartite NLS's. Like the NLS's in yeast transcription factor SW15, NLS2 contains a putative cdc 2 kinase site. However, unlike the case of SWI5, phosphorylation at this site did not affect the nuclear targeting of B‐Myb.