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Enhancement of phospholipase A 2 activation by phosphatidic acid endogenously formed through phospholipase D action in rat peritoneal mast cell
Author(s) -
Sato Takashi,
Ishimoto Tsuyoshi,
Akiba Satoshi,
Fujii Tatsuzo
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81440-b
Subject(s) - phosphatidic acid , liberation , phospholipase d , chemistry , phospholipase , mast cell , phospholipase a , biochemistry , phospholipase a2 , enzyme , biology , phospholipid , immunology , in vitro , membrane
Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA 2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p ‐bromophenacyl bromide. Upon exposure of the cells to ethanol in a high enough concentration to prevent PA formation, A23187‐induced arachidonate liberation was suppressed by 50% and the rest was completely inhibited by p ‐bromophenacyl bromide. In contrast, propranolol, which enhanced PA accumulation, significantly increased the arachidonate liberation. These results suggest that A23187‐induced PLA 2 activation may be potentiated, at least in part, by PA generated through PLD action.