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Expression cloning of an epithelial amiloride‐sensitive Na + channel
Author(s) -
Lingueglia Eric,
Voilley Nicolas,
Waldmann Rainer,
Lazdunski Michel,
Barbry Pascal
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81336-x
Subject(s) - amiloride , complementary dna , biology , epithelial sodium channel , transmembrane protein , acid sensing ion channel , peptide sequence , microbiology and biotechnology , ion channel , caenorhabditis elegans , gene , chemistry , genetics , sodium , receptor , organic chemistry
A complementary DNA encoding an amiloride‐sensitive Na + channel has been cloned and characterized from rat colon. The protein encoded by the cDNA has a sequence of 699 amino acids (79 kDa) containing several putative membrane spanning domains and potential phosphorylation sites. It forms a channel that has the electrophysiological and pharmacological properties characteristic of the epithelial Na + channel. Homologies (including in transmembrane domains) have been found between a part of the channel sequence and the Mec4 gene product of Caenorhabditis elegans , a protein associated with mutation‐induced neuronal degeneration.