Premium
Coupling of D 2 and D 3 dopamine receptors to G‐proteins
Author(s) -
Castro Sandra W.,
Strange Philip G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81168-y
Subject(s) - receptor , coupling (piping) , dopamine receptor , dopamine , chemistry , biochemistry , neuroscience , biology , materials science , metallurgy
Recombinant cell lines expressing D 2 and D 3 dopamine receptor isoforms have been used to study coupling of these receptors to G‐proteins from the effects of GTP to reduce agonist binding affinities in agonist/[ 3 H]spiperone competition experiments. D 2(short) expressed in Ltk − cells, D 2(long) expressed in Ltk − or CHO cells and D 3 expressed in CHO cells all showed coupling to the endogenous G‐proteins of the cells. The detailed agonist binding characteristics indicated that there were differences in receptor/G‐protein coupling for the same receptor (D 2(long) expressed in two cell types or for different receptors (D 2(long) ,D 2(short) expressed in the same cell type.