Premium
cDNAs sequences encoding cytochrome P450 (CYP71 family) from eggplant seedlings
Author(s) -
Umemoto Naoyuki,
Kobayashi Osamu,
Ishizaki-Nishizawa Osamu,
Toguri Toshihiro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80266-w
Subject(s) - gene , biology , cytochrome p450 , genetics , botany , biochemistry , enzyme
Three cytochrome P450 (P450) cDNAs were isolated from an eggplant hypocotyl cDNA library using eggplant CYP75 cDNA as a probe. These cDNAs have greater than 65% identity in their amino acid sequences, indicating that they belong to the same family. Comparison of these with P450 proteins from other sources showed that the protein with the greatest degree of homology is CYP71, isolated from avocado fruits (~ 48%). We concluded that they are novel members of the CYP71 gene family ( CYP71A2, 3 and 4 ). We have examined the level of mRNA transcripts from the CYP71 family in eggplant hypocotyl tissues and petunia flower buds, and found that the level of transcripts is developmentally regulated in the flower bud.