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Two‐dimensional 1 H NMR spectra of ferricytochrome c 551 from Pseudomonas aeruginosa
Author(s) -
Moratal José María,
Donaire Antonio,
Salgado Jesús,
Jiménez Hernias R.,
Castells Josep,
Piccioli Mario
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80140-p
Subject(s) - heme , chemistry , pseudomonas aeruginosa , proton nmr , methylene , cytochrome , hemeprotein , spectral line , redox , nmr spectra database , stereochemistry , nuclear magnetic resonance , crystallography , physics , biochemistry , inorganic chemistry , medicinal chemistry , bacteria , enzyme , biology , genetics , astronomy
The full assignment of 1 H NMR signals of heme proton resonances of ferricytochrome c 551 from Pseudomonas aeruginosa has been performed by means of 2D NMR experiments. This technique allows the complete and unequivocal assignment of all heme resonances, including methylene resonances of the propionic groups, directly implicated in the pH dependence of the redox properties of cytochrome c 551 .