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Tyrosine kinase activity is involved in the protein kinase C induced expression ofinterleukin‐1β gene in monocytic cells
Author(s) -
Palkama Tessa,
Matikainen Sampsa,
Hurme Mikko
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80045-v
Subject(s) - microbiology and biotechnology , protein kinase c , tyrosine kinase , mitogen activated protein kinase kinase , map2k7 , genistein , biology , reporter gene , chemistry , gene expression , cyclin dependent kinase 2 , protein kinase a , kinase , gene , biochemistry , signal transduction , endocrinology
The role of protein tyrosine kinases in the expression of interleukin‐1β (IL‐1β) gene in response to phorbol esters (PMA) in THP‐1 cell line was investigated. Genistein, a specific tyrosine kinase inhibitor, inhibited PMA induced IL‐1β protein and mRNA levels in THP‐1 cells. Genistein did not have a significant effect on PMA induced activity in transient transfection assays using reporter gene constructs containing the PMA responsive sequence of the IL‐ 1β gene linked to IL‐ 1β promoter, or five repeats of PMA responsive sites (AP‐1 sites) in front of a thymidine kinase promoter. This indicates that the tyrosine kinase activity required for the PMA induced IL‐1β expression is coupled downstream from or functions independent of the PMA induced AP‐1 activity.