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A new crystal form of the complex between seryl‐tRNA synthetase and tRNA Ser from Thermus thermophilus that diffracts to 2.8 Å resolution
Author(s) -
Yaremchuk A.D.,
Tukalo M.A.,
Krikliviy I.,
Malchenko N.,
Biou V.,
Berthet-Colominas C.,
Cusack S.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81319-h
Subject(s) - thermus thermophilus , dimer , orthorhombic crystal system , crystallography , transfer rna , monoclinic crystal system , stoichiometry , crystal structure , chemistry , stereochemistry , rna , escherichia coli , biochemistry , organic chemistry , gene
Two distinct complexes between seryl‐tRNA synthetase and tRNA Ser from Thermus themophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 Å, b = 126.8 Å, c = 197.1 Å, β = 132.4° and diffract to about 3.5 Å. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2 1 2 1 2 1 with unit cell dimensions a = 124.5 Å, b = 128.9 Å, c = 121.2 Å and diffract to 2.8 Å resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.
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