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Molecular cloning and sequence analysis of the cDNA encoding the human acrosin‐trypsin inhibitor (HUSI‐II)
Author(s) -
A. Moritz,
Hans Lilja,
Edwin Fink
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80099-o
Subject(s) - acrosin , complementary dna , peptide sequence , cdna library , microbiology and biotechnology , biology , signal peptide , open reading frame , sequence analysis , trypsin inhibitor , amino acid , nucleic acid sequence , trypsin , biochemistry , gene , genetics , enzyme , sperm , acrosome
A complete cDNA clone encoding the human acrosin‐trypsin inhibitor HUSI‐II has been isolated from a cDNA library of human testis and completely sequenced. The cDNA of 594 bp contained an open reading frame of 252 base pairs, The deduced amino acid sequence comprised the complete amino acid sequence of HUSI‐II[1] and a putative signal peptide. Northern blotting analysis revealed that HUSI‐II is synthesized in testis, epididymis and seminal vesicle, but not in the prostate gland.